Blocking type TSH receptor antibodies

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Blocking type anti-tSH receptor antibodies detected by radioreceptor assay in Graves' disease.

We previously developed a radioreceptor assay which is presumably specific for detection of blocking-type anti-TSH receptor (TSHR) antibodies using unsolubilized porcine TSHR. Employing this assay, we measured blocking TSH-binding inhibitory immunoglobulin (TBII) in the sera from 30 untreated Graves' patients and compared the results with those of a bioassay measuring thyroid stimulation blocki...

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Graves' Disease Mechanisms: The Role of Stimulating, Blocking, and Cleavage Region TSH Receptor Antibodies.

The immunologic processes involved in Graves' disease (GD) have one unique characteristic--the autoantibodies to the TSH receptor (TSHR)--which have both linear and conformational epitopes. Three types of TSHR antibodies (stimulating, blocking, and cleavage) with different functional capabilities have been described in GD patients, which induce different signaling effects varying from thyroid c...

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Bioassays for TSH Receptor Antibodies: Quo Vadis?

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Bioassays for TSH Receptor Antibodies: Quo Vadis?

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Bioassays for TSH Receptor Antibodies: Quo Vadis?

tations of GD [12] . TSHR bioassays show outstanding features. The biological activity of specific immunoglobulins is directly assessed on a fully functional TSHR holoreceptor expressed on intact live cells, a platform that is easily adaptable and tailored to detect Ab of specific function. The TSHR protein structure can be bioengineered and stably expressed in cell lines with protocols optimiz...

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ژورنال

عنوان ژورنال: Autoimmunity Highlights

سال: 2012

ISSN: 2038-0305,2038-3274

DOI: 10.1007/s13317-012-0028-1